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- KD/KO Validated
GANP Polyclonal antibody
GANP Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11054-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
11054-1-AP targets GANP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GANP fusion protein Ag1486 |
| Full Name | minichromosome maintenance complex component 3 associated protein |
| Calculated Molecular Weight | 218 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC013285 |
| Gene Symbol | MCM3AP |
| Gene ID (NCBI) | 8888 |
| RRID | AB_2266280 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GANP antibody 11054-1-AP | Download protocol |
| IHC protocol for GANP antibody 11054-1-AP | Download protocol |
| IF protocol for GANP antibody 11054-1-AP | Download protocol |
| IP protocol for GANP antibody 11054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.
| ||
J Immunol GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions to Reduce HIV-1 Infectivity.
| ||
Front Mol Neurosci The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.
| ||
J Biol Chem GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA. |
