GAPDH Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat, pig; other species not tested. |
| Cited species | human, monkey, mouse, rat, Sea cucumbers |
| Positive IHC detected in | human breast cancer |
| Positive IF detected in | Hela cells |
| Recommended dilution | IHC: 1:50-1:200 IF: 1:20-1:100 |
IP = Immunoprecipitation
Western blot of Hela cell with anti-GAPDH (10494-1-AP) at various dilutions.
IP result of anti-GAPDH (10494-1-AP for IP and Detection) with A549 cell lysate.
Immunohistochemistry of paraffin-embedded human breast cancer using 10494-1-AP(GAPDH Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using GAPDH antibody 10494-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | GAPDH fusion protein ag0766 | Full name | glyceraldehyde-3-phosphate dehydrogenase |
|
Calculated molecular weight |
36 kDa |
Observed molecular
weight |
36kd |
|
GenBank accession number |
BC004109 | Gene ID (NCBI) | 2597 |
| Gene symbol | GAPDH | Synonyms | G3PD; GAPD; MGC88685 |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, RNA binding, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a rabbit polyclonal antibody raised against full-length GAPDH of human origin. It can recognize the 36kDa GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
