GAPDH Antibody
Mouse Monoclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat, pig, zebrafish; other species not tested. |
| Cited species | human, mouse, rat |
| Positive WB detected in | Hela cells, HEK-293 cells, A549 cells, COLO 320 cells, HepG2 cells, human brain tissue, human colon tissue, human heart tissue, human kidney tissue, Jurkat cells, NIH/3T3 cells, Raji cells, SH-SY5Y cells, human testis tissue, RAW 264.7 cells, HT-1080 cells, zebrafish tissue |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:2000-1:20000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.
Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.
IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.
Hela cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig(GAPDH Antibody) at dilution of 1:8000
Immunohistochemistry of paraffin-embedded human breast cancer using 60004-1-Ig(GAPDH Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 60004-1-Ig(GAPDH Antibody) at Dilution 1:50 (under 40x lens)
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | GAPDH fusion protein ag0766 | Full name | glyceraldehyde-3-phosphate dehydrogenase |
|
Calculated molecular weight |
36 kDa |
Observed molecular
weight |
36kd |
|
GenBank accession number |
BC004109 | Gene ID (NCBI) | 2597 |
| Gene symbol | GAPDH | Synonyms | G3PD; GAPD; MGC88685 |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
