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WB analysis of human stomach using 14494-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 14494-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 14494-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 14494-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach cancer using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach cancer using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach cancer using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach using 14494-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 10x lens).

Gastrokine 1 Polyclonal antibody

Name: Gastrokine 1 Polyclonal antibody
Brand: Proteintech
SKU: 14494-1-AP

Gastrokine 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14494-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human stomach tissue, mouse stomach tissue, rat stomach tissue
Positive IHC detected in	human stomach tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14494-1-AP targets Gastrokine 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5898 Product name: Recombinant human GKN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-196 aa of BC059778 Sequence: NVNDDNNNAGSGQQSVSVNNEHNVANVDNNNGWDSWNSIWDYGNGFAATRLFQKKTCIVHKMNKEVMPSIQSLDALVKEKKLQGKGPGGPPPKGLMYSVNPNKVDDLSKFGKNIANMCRGIPTYMAEEMQEASLFFYSGTCYTTSVLWIVDISFCGDTVEN Predict reactive species
Full Name	gastrokine 1
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	20-22 kDa
GenBank Accession Number	BC059778
Gene Symbol	Gastrokine 1
Gene ID (NCBI)	56287
RRID	AB_10863653
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NS71
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1 may play an important role in normal gastric function and may be a gastric tumour suppressor.