GLS Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | mouse brain tissue, mouse kidney tissue |
| Positive IHC detected in | human gliomas |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
IP = Immunoprecipitation
IP result of anti-GLS (12855-1-AP for IP and Detection) with mouse brain tissue lysate.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12855-1-AP(GLS Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human gliomas using 12855-1-AP(GLS Antibody) at Dilution 1:100 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | GLS fusion protein ag3505 | Full name | glutaminase |
|
Calculated molecular weight |
669aa,73kd |
Observed molecular
weight |
65kd,58kd |
|
GenBank accession number |
BC038507 | Gene ID (NCBI) | 2744 |
| Gene symbol | GLS | Synonyms | AAD20; DKFZp686O15119; FLJ10358; GLS1; KIAA0838 |
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody is specific to KGA and GAC. |
