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GNAI2 Monoclonal antibody

GNAI2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F6H5

Cat no : 67007-1-Ig

Synonyms

GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAI2 Monoclonal antibody (67007-1-Ig)

    WB analysis using 67007-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using GNAI2 Monoclonal antibody (67007-1-Ig)

    WB analysis of U-937 using 67007-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GNAI2 Monoclonal antibody (67007-1-Ig)

    IF Staining of A431 using 67007-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 67007-1-Ig (GNAI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, U-937 cells, C6 cells, RAW 264.7 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67007-1-Ig targets GNAI2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityrabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNAI2 fusion protein Ag28560
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC012138
Gene Symbol GNAI2
Gene ID (NCBI) 2771
RRIDAB_2882324
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Protocols

Product Specific Protocols
WB protocol for GNAI2 antibody 67007-1-IgDownload protocol
IF protocol for GNAI2 antibody 67007-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rabbitWB

Genes (Basel)

GNAI2 Promotes Proliferation and Decreases Apoptosis in Rabbit Melanocytes.

Authors - Shuaishuai Hu
View Article