GREM2 Polyclonal antibody

GREM2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13892-1-AP

Synonyms

CKTSF1B2, DAN domain family member 3, DAND3, GREM2, Gremlin 2, PRDC



Tested Applications

Positive IHC detected inmouse ovary tissue, human ovary tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue, mouse ovary tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13892-1-AP targets GREM2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GREM2 fusion protein Ag4854
Full Name gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
Calculated Molecular Weight 168 aa, 19 kDa
GenBank Accession NumberBC046632
Gene Symbol GREM2
Gene ID (NCBI) 64388
RRIDAB_2877984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for GREM2 antibody 13892-1-APDownload protocol
IF protocol for GREM2 antibody 13892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Am J Pathol

The Onset of Heat-Induced Testicular Calcification in Mice: Involvement of the Telomeric Locus on Chromosome 1.

Authors - Masataka Chihara
humanIF

Stem Cells Dev

Coordinated proliferation and differentiation of human iPS cell-derived cardiac progenitor cells depends on BMP signaling regulation by Gremlin 2.

Authors - Jeffery Bylund
mouseIHC

Andrology

Genetic factors derived from the MRL/MpJ mouse function to maintain the integrity of spermatogenesis after heat exposure.

Authors - M Chihara
mouseWB,IHC

Metabolism

SGLT2 inhibitor empagliflozin downregulates miRNA-34a-5p and targets GREM2 to inactivate hepatic stellate cells and ameliorate non-alcoholic fatty liver disease-associated fibrosis

Authors - Yunfeng Shen