HDAC1 Antibody
Rabbit Polyclonal
Catalog number: 10197-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human pancreas cancer |
| Positive IF detected in | SH-SY5Y cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HepG2 cells were subjected to SDS PAGE followed by western blot with 10197-1-AP(HDAC1 Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human pancreas cancer using 10197-1-AP(HDAC1 Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of SH-SY5Y cells, using HDAC1 antibody 10197-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | HDAC1 fusion protein ag0256 | Full name | histone deacetylase 1 |
|
Calculated molecular weight |
55kd |
Observed molecular
weight |
60kd |
|
GenBank accession number |
BC000301 | Gene ID (NCBI) | 3065 |
| Gene symbol | HDAC1 | Synonyms | DKFZp686H12203; GON-10; HD1; RPD3; RPD3L1 |
Background
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC1. |
