HMOX1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IP |
| Cited applications | WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | mouse liver tissue, HeLa cells, HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 |
IP = Immunoprecipitation
IP result of HMOX1 antibody (10701-1-AP for IP and Detection) with HeLa cell lysate.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10701-1-AP(HMOX1 Antibody) at dilution of 1:600
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | HMOX1 fusion protein ag1190 | Full name | heme oxygenase (decycling) 1 |
|
Calculated molecular weight |
33kd |
Observed molecular
weight |
28-33kd |
|
GenBank accession number |
BC001491 | Gene ID (NCBI) | 3162 |
| Gene symbol | HMOX1 | Synonyms | HO-1; HSP32; bK286B10 |
Heme oxygenase (HO) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin IXa, carbon monoxide (CO), and iron .It has 3 isoforms:HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed(PMID:10194478 ).Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells(PMID:15451051 )and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, inflammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin(PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa)(James R. Reed,Pharmacology ,535-568).This antibody is specific to HMOX1. |
