Filter: AllWBIHCIF-PFC (Intra)

WB analysis of human plasma using 11541-1-AP
human plasma (0.3 ug) were subjected to SDS PAGE followed by western blot with 11541-1-AP (IgG light chain Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 11541-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 11541-1-AP (IgG light chain (lambda) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 11541-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 11541-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 11541-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IgG Lambda Light Chain antibody (11541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of Ramos using 11541-1-AP
1x10^6 Ramos cells were intracellularly stained with 0.4 ug IgG Lambda Light Chain Polyclonal antibody (11541-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Ig Lambda Light Chain Polyclonal antibody

Name: Ig Lambda Light Chain Polyclonal antibody
Brand: Proteintech
SKU: 11541-1-AP

Ig Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11541-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

IgG Lambda Light Chain, IGL, IgG, IGL-, immunoglobulin lambda locus

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Biotin CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594 HRP

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human plasma tissue, human spleen tissue
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue
Positive FC (Intra) detected in	Ramos cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11541-1-AP targets Ig Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2064 Product name: Recombinant human IGL- protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC020233 Sequence: MAWSPLLLTLLAHCTGSWAQSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYGNSNRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDSSLSGFVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS Predict reactive species
Full Name	immunoglobulin lambda locus
Calculated Molecular Weight	237 aa, 25 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC020233
Gene Symbol	IgG Lambda Light Chain
Gene ID (NCBI)	3535
RRID	AB_2877775
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the lambda light chain of human immunoglobulin.

Protocols

Product Specific Protocols
IF protocol for Ig Lambda Light Chain antibody 11541-1-AP	Download protocol
IHC protocol for Ig Lambda Light Chain antibody 11541-1-AP	Download protocol
WB protocol for Ig Lambda Light Chain antibody 11541-1-AP	Download protocol