Pre-IL18 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IP|
|Species specificity||human; other species not tested.|
|Positive WB detected in||HeLa cells|
|Positive IHC detected in||human colon cancer|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- IP result of anti-IL18 (10663-1-AP for IP and Detection) with HeLa cells lysate.
- HeLa cells were subjected to SDS PAGE followed by western blot with 10663-1-AP(Pre-IL18 Antibody) at dilution of 1:1000
- Immunohistochemistry of paraffin-embedded human colon cancer using 10663-1-AP(Pre-IL18 Antibody) at Dilution 1:100 (under 25x lens)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||Pre-IL18 fusion protein ag1063||Full name||interleukin 18 (interferon-gamma-inducing factor)|
|BC007461||Gene ID (NCBI)||3606|
|Gene symbol||IL18||Synonyms||IGIF; IL-18; IL-1g; IL1F4; MGC12320|
IL18 is a cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release interferon gamma which plays an important role in activating the macrophages or other cells. IL18 is synthesized as a 24kDa precursor and then cleaved into a biologically activate 18kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.