Pre-IL18 Antibody
Rabbit Polyclonal
Catalog number: 10663-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-IL18 (10663-1-AP for IP and Detection) with HeLa cells lysate.
HeLa cells were subjected to SDS PAGE followed by western blot with 10663-1-AP(Pre-IL18 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 10663-1-AP(Pre-IL18 Antibody) at Dilution 1:100 (under 25x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Pre-IL18 fusion protein ag1063 | Full name | interleukin 18 (interferon-gamma-inducing factor) |
|
Calculated molecular weight |
22kd |
Observed molecular
weight |
18-24 kDa |
|
GenBank accession number |
BC007461 | Gene ID (NCBI) | 3606 |
| Gene symbol | IL18 | Synonyms | IGIF; IL-18; IL-1g; IL1F4; MGC12320 |
Background
IL18 is a cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release interferon gamma which plays an important role in activating the macrophages or other cells. IL18 is synthesized as a 24kDa precursor and then cleaved into a biologically activate 18kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically. |
