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Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive FC (Intra) detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60319-1-Ig targets IL-1F10 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17250 Product name: Recombinant human IL-1F10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-152 aa of BC103966 Sequence: MCSLPMARYYIIKYADQKALYTRDGQLLVGDPVADNCCAEKICTLPNRGLDRTKVPIFLGIQGGSRCLACVETEEGPSLQLEDVNIEELYKGGEEATRFTFFQSSSGSAFRLEAAAWPGWFLCGPAEPQQPVQLTKESEPSARTKFYFEQSW Predict reactive species |
| Full Name | interleukin 1 family, member 10 (theta) |
| Calculated Molecular Weight | 152 aa, 17 kDa |
| GenBank Accession Number | BC103966 |
| Gene Symbol | IL1F10 |
| Gene ID (NCBI) | 84639 |
| RRID | AB_2881430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8WWZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1F10 antibody 60319-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
