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  • Featured Product
  • KD/KO Validated

IL-20RB Monoclonal antibody

IL-20RB Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 60221-1-Ig

Synonyms

DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-20RB Monoclonal antibody (60221-1-Ig)

    WB analysis using 60221-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60221-1-Ig (IL-20RB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-10A cells using IL-20RB Monoclonal antibody (60221-1-Ig)

    WB analysis of MCF-10A using 60221-1-Ig

    MCF-10A cells were subjected to SDS PAGE followed by western blot with 60221-1-Ig (IL-20RB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using IL-20RB Monoclonal antibody (60221-1-Ig)

    FC experiment of HeLa using 60221-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IL-20RB (60221-1-Ig, Clone:1A2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, MCF-10A cells, A375 cells, hTERT-RPE1 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60221-1-Ig targets IL-20RB in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-20RB fusion protein Ag14368
Full Name interleukin 20 receptor beta
Calculated Molecular Weight 311 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC033292
Gene Symbol IL20RB
Gene ID (NCBI) 53833
RRIDAB_11182930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-20 receptor subunit beta (IL20RB) is a single-pass type I membrane protein of the type II cytokine receptor family. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL-20-RI is a receptor for IL-19, IL-20 and IL-24. IL20RB can also form a heterodimer with IL22RA1. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.

Protocols

Product Specific Protocols
WB protocol for IL-20RB antibody 60221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.

Authors - Jia Li
  • KD Validated
View Article