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IL-22RA2 Polyclonal antibody

IL-22RA2 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 22861-1-AP

Synonyms

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using IL-22RA2 Polyclonal antibody (22861-1-AP)

    WB analysis of MDA-MB-453s using 22861-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22861-1-AP (IL22RA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IL-22RA2 Polyclonal antibody (22861-1-AP)

    IHC staining of human lung cancer using 22861-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IL-22RA2 Polyclonal antibody (22861-1-AP)

    IHC staining of human lung cancer using 22861-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-22RA2 Polyclonal antibody (22861-1-AP)

    IHC staining of human breast cancer using 22861-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-22RA2 Polyclonal antibody (22861-1-AP)

    IHC staining of human breast cancer using 22861-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using IL-22RA2 Polyclonal antibody (22861-1-AP)

    FC experiment of HeLa using 22861-1-AP

    1X10^6 HeLa cells were stained with 0.2ug IL22RA2 antibody (22861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Tested Applications

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22861-1-AP is IL-22RA2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-22RA2 fusion protein Ag18854
Full Name interleukin 22 receptor, alpha 2
Calculated Molecular Weight 263 aa, 31 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC125167
Gene Symbol IL22RA2
Gene ID (NCBI) 116379
RRIDAB_2879180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-22 receptor subunit alpha-2 (IL22RA2, also known as CRF2-10) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. It has been described that human IL22RA2 gene encodes three alternatively spliced isoforms called CRF2-10L, CRF2-10, and CRF2-10s, with molecular weights of 31 kDa, 27 kDa, and 15 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for IL-22RA2 antibody 22861-1-APDownload protocol
IHC protocol for IL-22RA2 antibody 22861-1-APDownload protocol
FC protocol for IL-22RA2 antibody 22861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols