IRF5 Antibody
Rabbit Polyclonal
Catalog number: 10547-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | IHC, IP, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | Jurkat cells, A549 cells, HeLa cells, human heart tissue, NIH/3T3 cells, SH-SY5Y cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP(IRF5 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 10547-1-AP(IRF5 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 10547-1-AP(IRF5 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | IRF5 fusion protein ag0841 | Full name | interferon regulatory factor 5 |
|
Calculated molecular weight |
56kd |
Observed molecular
weight |
47-50kd |
|
GenBank accession number |
BC004201 | Gene ID (NCBI) | 3663 |
| Gene symbol | IRF5 | Synonyms | SLEB10 |
Background
IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5. |
