ISG20L2 Polyclonal antibody

ISG20L2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24639-1-AP

Synonyms

HSD 38, ISG20L2



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

24639-1-AP targets ISG20L2 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISG20L2 fusion protein Ag20026
Full Name interferon stimulated exonuclease gene 20kDa-like 2
Calculated Molecular Weight 353 aa, 39 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000575
Gene Symbol ISG20L2
Gene ID (NCBI) 81875
RRIDAB_2879649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISG20L2, also named HSD38, is a member of a vertebrate exonucleases family. ISG20L2 is composed of at least two functional domains, one at its N-terminal half allowing intracellular localization and association with the binding partners and the other at its C-terminal half bearing the 3` to 5` exoribonuclease activity. The molecular weight of ISG20L2 is 39 kDa (PMID: 18065403).

Protocols

Product Specific Protocols
WB protocol for ISG20L2 antibody 24639-1-APDownload protocol
IP protocol for ISG20L2 antibody 24639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JCI Insight

ISG20L2 suppresses bortezomib anti-myeloma activity by attenuating bortezomib binding to PSMB5

Authors - Yan Yang
humanWB

Nucleic Acids Res

Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA

Authors - Yinliang Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sara (Verified Customer) (05-24-2023)

It is the best ISG20L2 I have tried by far compared to other commercial ones. I have used it for WB (1:5000) and for immunoprecipitation. I need to mention that we tried it for IF but it did not work properly, at least for Jurkat T cells!

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:5000