Featured Product
KD/KO Validated

KATNB1 Polyclonal antibody

KATNB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14969-1-AP

Publications (14)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KAT, Katanin p80 subunit B1, Katanin p80 WD40 repeat-containing subunit B1, p80 katanin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis using 14969-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 14969-1-AP
WB result of KATNB1 antibody (14969-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KATNB1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse skeletal muscle using 14969-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 14969-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 14969-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 14969-1-AP
IP result of anti-KATNB1 (IP:14969-1-AP, 3ug; Detection:14969-1-AP 1:500) with HeLa cells lysate 3000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse lung using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14969-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14969-1-AP (KATNB1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 14969-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14969-1-AP (KATNB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for KATNB1 in ?

Overview

Tested Applications
Product Information
Publications (14)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, mouse testis tissue, mouse stomach tissue, mouse kidney tissue, mouse lung tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14969-1-AP targets KATNB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6836 Product name: Recombinant human KATNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 305-655 aa of BC001353 Sequence: VTRTGTVARDPVQDHRPLAQPLPNPSAPLRRIYERPSTTCSKPQRVKQNSESERRSPSSEDDRDERESRAEIQNAEDYNEIFQPKNSISRTPPRRSEPFPAPPEDDAATAKEAAKPSPAMDVQFPVPNLEVLPRPPVVASTPAPKAEPAIIPATRNEPIGLKASDFLPAVKIPQQAELVDEDAMSQIRKGHDTMCVVLTSRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVVVDLLNIVNQKASLWKLDLCTTVLPQIEKLLQSKYESYVQTGCTSLKLILQRFLPLITDMLAAPPSVGVDISREERLHKCRLCYKQLKSISGLVKSKSGLSGRHGSTFRELHLLMASLD Predict reactive species
Full Name	katanin p80 (WD repeat containing) subunit B 1
Calculated Molecular Weight	72 kDa
Observed Molecular Weight	72-80 kDa
GenBank Accession Number	BC001353
Gene Symbol	KATNB1
Gene ID (NCBI)	10300
RRID	AB_10637861
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BVA0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Katanin p80 WD40-containing subunit B1 is a protein that in humans is encoded by the KATNB1 gene. Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.

Protocols

Product Specific Protocols
IF protocol for KATNB1 antibody 14969-1-AP	Download protocol
IHC protocol for KATNB1 antibody 14969-1-AP	Download protocol
IP protocol for KATNB1 antibody 14969-1-AP	Download protocol
WB protocol for KATNB1 antibody 14969-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-KATNB1 (Katanin p80) antibody (Cat# 14969-1-AP) has 14 citations across journals including Nature Cell Biology, Neuron, Molecular Cell, Developmental Cell, Cell Reports, Journal of Cell Biology, Structure, Journal of Neuroscience, and FASEB Journal. Research fields span spindle architecture, microtubule dynamics, centriole/cilia biogenesis, synaptic plasticity, drug discovery targeting katanin, and host-pathogen interactions involving Klebsiella pneumoniae.

Species	Application	Title
mouse	WB	Nat Cell Biol Cell state-specific cytoplasmic density controls spindle architecture and scaling Authors - Tobias Kletter View Article
mouse	WB,IF	Neuron Katanin p80 regulates human cortical development by limiting centriole and cilia number. Authors - Wen F Hu View Article
mouse	WB,IP,IHC,IF	Mol Cell PDCD5 promotes substrate release from the TRiC complex in cilia and flagella. Authors - Huafang Wei View Article
human	WB	Dev Cell Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition. Authors - Kai Jiang View Article
	WB	Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals. Authors - Ana C Almeida KD Validated View Article
human	WB	Eur J Med Chem Design, synthesis, antitumor activities and biological studies of novel diaryl substituted fused heterocycles as dual ligands targeting tubulin and katanin. Authors - Feng Gao View Article