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  • KD/KO Validated

KATNB1 Polyclonal antibody

KATNB1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14969-1-AP

Synonyms

KAT, Katanin p80 subunit B1, KATNB1, p80 katanin



Tested Applications

Positive WB detected inA431 cells, mouse skeletal muscle tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14969-1-AP targets KATNB1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KATNB1 fusion protein Ag6836
Full Name katanin p80 (WD repeat containing) subunit B 1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72-80 kDa
GenBank Accession NumberBC001353
Gene Symbol KATNB1
Gene ID (NCBI) 10300
RRIDAB_10637861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Katanin p80 WD40-containing subunit B1 is a protein that in humans is encoded by the KATNB1 gene. Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.

Protocols

Product Specific Protocols
WB protocol for KATNB1 antibody 14969-1-APDownload protocol
IF protocol for KATNB1 antibody 14969-1-APDownload protocol
IP protocol for KATNB1 antibody 14969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IF

Neuron

Katanin p80 regulates human cortical development by limiting centriole and cilia number.

Authors - Wen F Hu
humanWB

Dev Cell

Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition.

Authors - Kai Jiang
humanIF, WB

J Cell Biol

WDR62 localizes katanin at spindle poles to ensure synchronous chromosome segregation.

Authors - Amanda Guerreiro
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
mouseIF

J Neurosci

Functional inhibition of katanin affects synaptic plasticity

Authors - Franco L Lombino
humanWB

Structure

Structural Basis of Formation of the Microtubule Minus-End-Regulating CAMSAP-Katanin Complex.

Authors - Kai Jiang
  • KO Validated