Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, MDCK cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13105-1-AP targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3748 Product name: Recombinant human KIF2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 328-679 aa of BC031828 Sequence: YKKLELQVYATFFEIYSGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRKGKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVDPTAAGDVRPIMHHPPNQIDDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSYATQLEAILEQKIDILTELRDKVKSFRAALQEEEQASKQINPKRPRAL Predict reactive species |
| Full Name | kinesin heavy chain member 2A |
| Calculated Molecular Weight | 679 aa, 77 kDa |
| Observed Molecular Weight | 80-100 kDa |
| GenBank Accession Number | BC031828 |
| Gene Symbol | KIF2A |
| Gene ID (NCBI) | 3796 |
| RRID | AB_2265336 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00139 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Kinesin family member 2a (KIF2A), one of the kinesin family members, is identified in mammalian cells and involved in regulating microtubule dynamics during the mitosis via modulating intracellular transport, cell division, and bipolar spindle assembly (PMID: 17538014). KIF2A is essential for embryonic and postnatal brain development and maintenance. During brain development, regulation of MT dynamics by KIF2A is required for neuronal migration, polarization, and connectivity (PMID: 37488893). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma (PMID: 32106376, PMID: 26070867, PMID: 26602436).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KIF2A antibody 13105-1-AP | Download protocol |
| IF protocol for KIF2A antibody 13105-1-AP | Download protocol |
| IHC protocol for KIF2A antibody 13105-1-AP | Download protocol |
| IP protocol for KIF2A antibody 13105-1-AP | Download protocol |
| WB protocol for KIF2A antibody 13105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
KIF2A Antibody (Cat# 13105-1-AP) has 21 citations published in journals including Science, EMBO J, Nucleic Acids Res, Cell Reports, Molecular Therapy, J Cell Biol, FASEB J, Cell Insight, and others. Research fields span cell biology (spindle assembly, microtubule dynamics, centrosome function), oncology (gastric, lung, liver, and oral cancers), reproductive biology (oocyte meiosis), neuroscience (synapse dysfunction, epilepsy), immunology (inflammatory arthritis), and drug discovery (tubulin-targeting ligands).
| Species | Application | Title |
|---|---|---|
Int J Oral Sci RGS12 represses oral squamous cell carcinoma by driving M1 polarization of tumor-associated macrophages via controlling ciliary MYCBP2/KIF2A signaling | ||
Nucleic Acids Res KIF2A-mediated microtubule-dependent nuclear envelope invagination drives nonhomologous end joining.
| ||
Mol Ther Targeting S100A8/A9-NCF1 Axis in Tumor microenvironment to Prevent Tumor Metastasis by Self-assembled Peptide Nanofibers | ||
EMBO J The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network
|
Reviews
What customers say
One reviewer (Dorota S.) rated this antibody 4 out of 5 stars for Western Blot on U2OS cells using a dilution range of 1:250–1:1000. She described it as "good for western blotting." Overall, the single review reflects a positive but not perfect experience with the product for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dorota (Verified Customer) (04-10-2019) | Good for western blotting.
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