KRT14 Antibody
Rabbit Polyclonal
Catalog number: 10143-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IF, IHC |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | mouse |
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human cervix |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP(KRT14 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervix using 10143-1-AP(KRT14 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervix using 10143-1-AP(KRT14 Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using KRT14 antibody 10143-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | KRT14 fusion protein ag0188 | Full name | keratin 14 |
|
Calculated molecular weight |
472aa,52kd |
Observed molecular
weight |
47-50kd |
|
GenBank accession number |
BC002690 | Gene ID (NCBI) | 3861 |
| Gene symbol | KRT14 | Synonyms | CK14; EBS3; EBS4; K14; NFJ |
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. |
