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  • Featured Product
  • KD/KO Validated

LARP1 Polyclonal antibody

LARP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, drosophila

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13708-1-AP

Synonyms

KIAA0731, La related protein 1, LARP, LARP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of HEK-293T using 13708-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of mouse brain using 13708-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of HeLa using 13708-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of HepG2 using 13708-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of mouse heart using 13708-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using LARP1 Polyclonal antibody (13708-1-AP)

    WB analysis of mouse testis using 13708-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human liver cancer using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human ovary tumor using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human ovary tumor using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LARP1 Polyclonal antibody (13708-1-AP)

    IHC staining of human liver cancer using 13708-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LARP1 Polyclonal antibody (13708-1-AP)

    IF Staining of HepG2 using 13708-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13708-1-AP (LARP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, mouse heart tissue, mouse testis tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13708-1-AP targets LARP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, drosophila samples.

Tested Reactivity human, mouse, rat, drosophila
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LARP1 fusion protein Ag4645
Full Name La ribonucleoprotein domain family, member 1
Calculated Molecular Weight 1096 aa, 124 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC033856
Gene Symbol LARP1
Gene ID (NCBI) 23367
RRIDAB_2296670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LARP1 antibody 13708-1-APDownload protocol
IHC protocol for LARP1 antibody 13708-1-APDownload protocol
IF protocol for LARP1 antibody 13708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.

Authors - Pedro Fuentes
  • KO Validated
View Article
humanWB

Mol Cell Proteomics

A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications

Authors - Yuan-Qin Min
View Article
mouseWB

Mol Autism

Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.

Authors - Annie Hien
View Article