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LSM5 Polyclonal antibody

LSM5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10700-1-AP

Synonyms

LSM5, YER146W

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LSM5 Polyclonal antibody (10700-1-AP)

    WB analysis of HeLa using 10700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LSM5 Polyclonal antibody (10700-1-AP)

    WB analysis using 10700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using LSM5 Polyclonal antibody (10700-1-AP)

    WB analysis of human placenta using 10700-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LSM5 Polyclonal antibody (10700-1-AP)

    WB analysis of human brain using 10700-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LSM5 Polyclonal antibody (10700-1-AP)

    WB analysis of HL-60 using 10700-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using LSM5 Polyclonal antibody (10700-1-AP)

    WB analysis of mouse ovary using 10700-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LSM5 Polyclonal antibody (10700-1-AP)

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LSM5 Polyclonal antibody (10700-1-AP)

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LSM5 Polyclonal antibody (10700-1-AP)

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LSM5 Polyclonal antibody (10700-1-AP)

    IF Staining of HeLa using 10700-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10700-1-AP (LSM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue, human placenta tissue, mouse ovary tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10700-1-AP targets LSM5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM5 fusion protein Ag1145
Full Name LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC005938
Gene Symbol LSM5
Gene ID (NCBI) 23658
RRIDAB_2138475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LSM5 antibody 10700-1-APDownload protocol
IHC protocol for LSM5 antibody 10700-1-APDownload protocol
IF protocol for LSM5 antibody 10700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols