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LSR Polyclonal antibody

LSR Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 18216-1-AP

Synonyms

ILDR3, LISCH, LISCH7, LSR

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using LSR Polyclonal antibody (18216-1-AP)

    WB analysis of HT-29 using 18216-1-AP

    HT-29 cells (LSR+) and HeLa cells (LSR-) were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LSR Polyclonal antibody (18216-1-AP)

    WB analysis using 18216-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse stomach using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse stomach using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse liver using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse liver using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using LSR Polyclonal antibody (18216-1-AP)

    FC experiment of HepG2 using 18216-1-AP

    1X10^6 HepG2 cells were stained with .2ug LSR antibody (18216-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse stomach tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18216-1-AP targets LSR in WB, IHC, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSR fusion protein Ag12961
Full Name lipolysis stimulated lipoprotein receptor
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 60-70 kDa, 56 kDa
GenBank Accession NumberBC004381
Gene Symbol LSR
Gene ID (NCBI) 51599
RRIDAB_2250137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).

Protocols

Product Specific Protocols
WB protocol for LSR antibody 18216-1-APDownload protocol
IHC protocol for LSR antibody 18216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biosens Bioelectron

A multiple mixed TiO2 mesocrystal junction based PEC-colorimetric immunoassay for specific recognition of lipolysis stimulated lipoprotein receptor.

Authors - Yanjie Chen
View Article
mouseWB

J Hazard Mater

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Authors - Zijun Peng
View Article