Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20331-1-AP targets LTBR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14065 Product name: Recombinant human LTBR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-435 aa of BC026262 Sequence: KSHPSLCRKLGSLLKRRPQGEGPNPVAGSWEPPKAHPYFPDLVQPLLPISGDVSPVSTGLPAAPVLEAGVPQQQSPLDLTREPQLEPGEQSQVAHGTNGIHVTGGSMTITGNIYIYNGPVLGGPPGPGDLPATPEPPYPIPEEGDPGPPGLSTPHQEDGKAWHLAETEHCGATPSNRGPRNQFITHD Predict reactive species |
| Full Name | lymphotoxin beta receptor (TNFR superfamily, member 3) |
| Calculated Molecular Weight | 435 aa, 47 kDa |
| Observed Molecular Weight | 47-60 kDa |
| GenBank Accession Number | BC026262 |
| Gene Symbol | LTBR |
| Gene ID (NCBI) | 4055 |
| RRID | AB_10694276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36941 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lymphotoxin-beta receptor (LTBR) is a receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. It can activate the NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA1-LTB2) (PMID: 24248355). LTBR is constitutively expressed on stromal cells and myeloid lineage cells but not on T or B lymphocytes, which may be a novel immune checkpoint of tumor-associated macrophages (PMID: 20603617; 39429877).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for LTBR antibody 20331-1-AP | Download protocol |
| IF protocol for LTBR antibody 20331-1-AP | Download protocol |
| IHC protocol for LTBR antibody 20331-1-AP | Download protocol |
| WB protocol for LTBR antibody 20331-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-LTβR antibody (Cat# 20331-1-AP) has accumulated 16 citations across journals including Cancer Cell, Nature Biotechnology, Nature Communications, Advanced Science, Cell Death & Differentiation, Molecular Therapy, Theranostics, Journal of Immunology, and American Journal of Pathology. Associated research fields encompass oncology (gastric, bladder, glioma, head and neck cancers), immunology, fibrosis, diabetes, inflammation, and T cell signaling.
| Species | Application | Title |
|---|---|---|
Cancer Cell B cells disrupt tertiary lymphoid structure formation and suppress anti-tumor immunity. | ||
Nat Commun Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury | ||
Theranostics Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. | ||
Adv Sci (Weinh) Plac1+ Tumor Cell-Treg Interplay Supports Tumorigenesis and Progression of Head and Neck Cancer | ||
Adv Sci (Weinh) Radiation-Induced Tumor-Intrinsic LTβR N-Glycosylation Suppresses Pyroptosis Through TRIM28-Mediated PCBP2 SUMOylation to Promote Gastric Cancer Radioresistance |
