LYPLA1 Antibody
Rabbit Polyclonal
Catalog number: 16055-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 16055-1-AP(LYPLA1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 16055-1-AP(LYPLA1 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer using 16055-1-AP(LYPLA1 Antibody) at Dilution 1:100 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | LYPLA1 fusion protein ag9022 | Full name | lysophospholipase I |
|
Calculated molecular weight |
230aa,25kd |
Observed molecular
weight |
25 kDa |
|
GenBank accession number |
BC010397 | Gene ID (NCBI) | 10434 |
| Gene symbol | LYPLA1 | Synonyms | APT-1; LPL1; LYSOPLA |
Background
LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193). This antibody is specific to LYPLA1. |
