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MAGED1 Polyclonal antibody

MAGED1 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11539-1-AP

Synonyms

DLXIN 1, MAGE D1 antigen, MAGE tumor antigen CCF, MAGED1, melanoma antigen family D, 1, Melanoma associated antigen D1, NRAGE, PP2250

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAGED1 Polyclonal antibody (11539-1-AP)

    WB analysis of HEK-293 using 11539-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MAGED1 Polyclonal antibody (11539-1-AP)

    WB analysis of human brain using 11539-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Apoptosised HeLa cells using MAGED1 Polyclonal antibody (11539-1-AP)

    WB analysis of Apoptosised HeLa using 11539-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAGED1 Polyclonal antibody (11539-1-AP)

    WB analysis of HeLa using 11539-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAGED1 Polyclonal antibody (11539-1-AP)

    WB analysis of HEK-293 using 11539-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MAGED1 Polyclonal antibody (11539-1-AP)

    IHC staining of human gliomas using 11539-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11539-1-AP (MAGED1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MAGED1 Polyclonal antibody (11539-1-AP)

    IHC staining of human gliomas using 11539-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11539-1-AP (MAGED1 Antibody) at dilution of 1:100 (under 40x lens).

  • Flow cytometry (FC) experiment of HeLa cells using MAGED1 Polyclonal antibody (11539-1-AP)

    FC experiment of HeLa using 11539-1-AP

    1X10^6 HeLa cells were stained with 0.2ug MAGED1 antibody (11539-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHEK-293 cells, Apoptosised HeLa cells, HeLa cells, human brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11539-1-AP targets MAGED1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGED1 fusion protein Ag2109
Full Name melanoma antigen family D, 1
Calculated Molecular Weight86 kDa; 92 kDa
Observed Molecular Weight 66 kDa, 92-95 kDa
GenBank Accession NumberBC014070
Gene Symbol MAGED1
Gene ID (NCBI) 9500
RRIDAB_2138545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527).

Protocols

Product Specific Protocols
WB protocol for MAGED1 antibody 11539-1-APDownload protocol
IHC protocol for MAGED1 antibody 11539-1-APDownload protocol
FC protocol for MAGED1 antibody 11539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC,IF,IP

Sci Rep

Dynamic expression of Mage-D1 in rat dental germs and potential role in mineralization of ectomesenchymal stem cells

Authors - Meng Li
View Article