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MAGED1 Polyclonal antibody

Name: MAGED1 Polyclonal antibody
Brand: Proteintech
SKU: 22053-1-AP

MAGED1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22053-1-AP

Synonyms

DLXIN 1, MAGE D1 antigen, MAGE tumor antigen CCF, MAGED1, melanoma antigen family D, 1, Melanoma associated antigen D1, NRAGE, PP2250

Validation Data Gallery

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WB analysis of A549 using 22053-1-AP
WB result of MAGED1 antibody (22053-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAGED1 transfected A549 cells.
WB analysis of A549 using 22053-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22053-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22053-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 22053-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22053-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 22053-1-AP
IP result of anti-MAGED1 (IP:22053-1-AP, 4ug; Detection:22053-1-AP 1:1000) with A549 cells lysate 400ug.
IHC staining of mouse placenta using 22053-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 22053-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 22053-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 22053-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 22053-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 22053-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 22053-1-AP
Immunofluorescent analysis of A549 cells using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 22053-1-AP
1X10^6 HeLa cells were stained with .2ug MAGED1 antibody (22053-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Cat No. 22053-1-AP

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Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, mouse placenta tissue
Positive IP detected in	A549 cells
Positive IHC detected in	mouse placenta tissue, human skin tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A549 cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)	IF : 1:20-1:200
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

22053-1-AP targets MAGED1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAGED1 fusion protein Ag17047
Full Name	melanoma antigen family D, 1
Calculated Molecular Weight	834 aa, 92 kDa
Observed Molecular Weight	95-100 kDa
GenBank Accession Number	BC014070
Gene Symbol	MAGED1
Gene ID (NCBI)	9500
RRID	AB_11182601
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527). Three transcript variants encoding two different isoforms (86kDa and 92 kDa) have been found for the gene of MAGED1.

Protocols

Product Specific Protocols
WB protocol for MAGED1 antibody 22053-1-AP	Download protocol
IHC protocol for MAGED1 antibody 22053-1-AP	Download protocol
IF protocol for MAGED1 antibody 22053-1-AP	Download protocol
IP protocol for MAGED1 antibody 22053-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Clin Transl Med Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice. Authors - Tingting Qin View Article
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system. Authors - Qiang Lu View Article
human	WB,IHC,IF	Tumour Biol Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells. Authors - Huandi Zhou KD Validated View Article
human	WB,IHC	Gene NRAGE confers poor prognosis and promotes proliferation, invasion, and chemoresistance in gastric cancer. Authors - Xiaodi Jiang KD Validated View Article

MAGED1 Polyclonal antibody

MAGED1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of A549 using 22053-1-AP

WB analysis of A549 using 22053-1-AP

WB analysis using 22053-1-AP

WB analysis of HEK-293 using 22053-1-AP

WB analysis of mouse placenta using 22053-1-AP

WB analysis of HEK-293 using 22053-1-AP

IP experiment of A549 using 22053-1-AP

IHC staining of mouse placenta using 22053-1-AP

IHC staining of mouse placenta using 22053-1-AP

IHC staining of human placenta using 22053-1-AP

IHC staining of human placenta using 22053-1-AP

IHC staining of human skin using 22053-1-AP

IHC staining of human skin using 22053-1-AP

IF Staining of A549 using 22053-1-AP

FC experiment of HeLa using 22053-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice.

Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.

Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.

NRAGE confers poor prognosis and promotes proliferation, invasion, and chemoresistance in gastric cancer.