Tested Applications
| Positive WB detected in | HeLa cells, Chloroquine treated HeLa cells |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18725-1-AP targets LC3B-Specific in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | microtubule-associated protein 1 light chain 3 beta |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15 kDa, 18 kDa |
| GenBank Accession Number | NM_022818 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_2137745 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
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Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| IF protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| IHC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| WB protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LC3B-Specific Polyclonal antibody (Cat# 18725-1-AP) has 493 total citations spanning journals including Nature, Autophagy, Cell Death & Disease, Advanced Science, Biomaterials, and International Journal of Molecular Sciences. Research fields encompass autophagy and mitophagy, cancer biology, neurodegenerative diseases, cardiovascular disorders, diabetes, ferroptosis, mitochondrial biology, inflammation, and environmental toxicology.
| Species | Application | Title |
|---|---|---|
Cell Res Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models. | ||
J Extracell Vesicles Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles. | ||
Am J Respir Crit Care Med Acid Sphingomyelinase Inhibition Attenuates Cell Death in Mechanically-Ventilated Newborn Rat Lung. | ||
Nat Neurosci The mitochondrial unfolded protein response in human microglia disrupts neuronal-glial communication and promotes senescence. | ||
Autophagy An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles. |
Reviews
What customers say
Users report mixed results. Several reviewers found the antibody effective for Western blotting in cell lines and mouse brain tissue, with one noting strong IHC labeling in primary rat cortical neurons. However, others experienced issues: one reported strong non-specific bands above 20 kDa that obscured the LC3B signal, and another found only weak detection in cell lysates and nearly no signal in extracellular vesicles. Overall, performance appears sample-dependent, working well in some contexts but showing limitations in others.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jacob (Verified Customer) (07-23-2026) | Has a very strong non-specific banding pattern above 20kDa, so the LC3B was faint. 2*
 |
Alicia (Verified Customer) (09-30-2025) | Control microglia derived from iPSC with and without bafilomycin. Drug treated microglia derived from iPSC with and without bafilomycin.
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Allegra (Verified Customer) (09-30-2025) | Work for cell lysates only in concentration 1:500 and this is only slightly detectable. In extracellular vesicles nearly no signal.
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Azita (Verified Customer) (06-02-2021) | Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:300. Immunohistochemistry labelling of primary rat cortical neurons using LC3B-Specific Polyclonal antibody at dilution of 1:500 showed strong labelling.
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Tanusree (Verified Customer) (12-03-2019) | The antibody works great in Western blotting analysis.
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