MAT1A Antibody
Rabbit Polyclonal
Catalog number: 12395-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | mouse liver tissue, HepG2 cells |
| Recommended dilution | WB: 1:1000-1:10000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12395-1-AP(MAT1A Antibody) at dilution of 1:2000
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | MAT1A fusion protein ag3076 | Full name | methionine adenosyltransferase I, alpha |
|
Calculated molecular weight |
395aa,44kd |
Observed molecular
weight |
44-55kd |
|
GenBank accession number |
BC018359 | Gene ID (NCBI) | 4143 |
| Gene symbol | MAT1A | Synonyms | MAT; MATA1; SAMS; SAMS1 |
Background
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and may cross-react with MAT2A. Western blot analysis using this antibody detected a single band around 44-50kD in liver. |
