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MUPCDH Polyclonal antibody

MUPCDH Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25619-1-AP

Synonyms

CDHR5, MU PCDH, Mu protocadherin, MUCDHL, mucin like protocadherin, MUPCDH

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MUPCDH Polyclonal antibody (25619-1-AP)

    WB analysis of HEK-293 using 25619-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25619-1-AP (MUPCDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MUPCDH Polyclonal antibody (25619-1-AP)

    IHC staining of human kidney using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MUPCDH Polyclonal antibody (25619-1-AP)

    IHC staining of human kidney using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MUPCDH Polyclonal antibody (25619-1-AP)

    IHC staining of human small intestine using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MUPCDH Polyclonal antibody (25619-1-AP)

    IHC staining of human small intestine using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25619-1-AP is MUPCDH Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUPCDH fusion protein Ag22537
Full Name mucin-like protocadherin
Calculated Molecular Weight 845 aa, 88 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC038405
Gene Symbol MUPCDH
Gene ID (NCBI) 53841
RRIDAB_2880164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUPCDH (Mu-protocadherin), also known as MUCDHL (Mucin and cadherin-like protein) and CDHR5 (Cadherin-related family member 5), is a member of the cadherin superfamily. It is involved in calcium-dependent cell adhesion and may have additional functions in terminally differentiated epithelia. The MUPCDH gene maps to 11p15.5, with highest expression in kidney, liver, colon and small intestine. Alternatively spliced transcript variants encoding different isoforms have been described. (PMID: 12167596; 21839066)

Protocols

Product Specific Protocols
WB protocol for MUPCDH antibody 25619-1-APDownload protocol
IHC protocol for MUPCDH antibody 25619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols