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Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, HEK-293 cells, HT-1080 cells, A431 cells, mouse thymus tissue, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human ovary tissue, rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
14898-1-AP targets NAP1L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NAP1L1 fusion protein Ag6690 Predict reactive species |
| Full Name | nucleosome assembly protein 1-like 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC002387 |
| Gene Symbol | NAP1L1 |
| Gene ID (NCBI) | 4673 |
| RRID | AB_2150696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55209 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IHC protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IF protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IP protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Reprod Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage | ||
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.
| ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
J Virol NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation. | ||
Cancer Cell Int NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.
| ||
J Dermatol Sci Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells. |
