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  • KD/KO Validated

NAP1L1 Polyclonal antibody

NAP1L1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14898-1-AP

Synonyms

hNRP, NAP 1 related protein, NAP1, NAP1L, NAP1L1, NRP



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HT-1080 cells, A431 cells, HEK-293 cells, mouse thymus tissue, HL-60 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14898-1-AP targets NAP1L1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAP1L1 fusion protein Ag6690
Full Name nucleosome assembly protein 1-like 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC002387
Gene Symbol NAP1L1
Gene ID (NCBI) 4673
RRIDAB_2150696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NAP1L1 antibody 14898-1-APDownload protocol
IHC protocol for NAP1L1 antibody 14898-1-APDownload protocol
IF protocol for NAP1L1 antibody 14898-1-APDownload protocol
IP protocol for NAP1L1 antibody 14898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.

Authors - Ye-Wei Zhang
  • KD Validated
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang
mouseIF,WB

J Virol

NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.

Authors - Francisco Dominguez
humanWB,IHC,IF

Cancer Cell Int

NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.

Authors - Shu Liu
  • KD Validated
humanWB

J Dermatol Sci

Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.

Authors - Satoru Mizuhashi
humanWB,IF

Cytoskeleton (Hoboken)

NAP1L1 is a novel microtubule-associated protein

Authors - Nannan Peng