NEFL Antibody
Rabbit Polyclonal
Catalog number: 12998-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human brain |
| Positive IF detected in | NIH3T3 cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
WB result of 12998-1-AP.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12998-1-AP(NEFL Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human brain using 12998-1-AP(NEFL Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain using 12998-1-AP(NEFL Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of NIH3T3 cells, using NEFL antibody 12998-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | NEFL fusion protein ag3667 | Full name | neurofilament, light polypeptide |
|
Calculated molecular weight |
543aa,62kd |
Observed molecular
weight |
65-68kd,140-160kd |
|
GenBank accession number |
BC039237 | Gene ID (NCBI) | 4747 |
| Gene symbol | NEFL | Synonyms | CMT1F; CMT2E; FLJ53642; NF-L; NF68; NFL |
Background
NEFL, also named as NF68 and NFL, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd.This antibody is specific to NEFL. It has a very weak reaction to NEFM. |
