Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, human brain tissue, human colon tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25805-1-AP targets NF-M in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22709 Product name: Recombinant human NEFM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-97 aa of BC002421 Sequence: MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLS Predict reactive species |
| Full Name | neurofilament, medium polypeptide |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC002421 |
| Gene Symbol | NF-M |
| Gene ID (NCBI) | 4741 |
| RRID | AB_2880245 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07197 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognizes endogenous NF-M protein.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NF-M antibody 25805-1-AP | Download protocol |
| IF protocol for NF-M antibody 25805-1-AP | Download protocol |
| IHC protocol for NF-M antibody 25805-1-AP | Download protocol |
| WB protocol for NF-M antibody 25805-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NF-M Polyclonal antibody (Cat# 25805-1-AP) has 17 citations published in journals including Nature Chemical Biology, Bioactive Materials, Theranostics, Journal of Neuroinflammation, Biomaterials Science, Molecular Autism, International Journal of Molecular Sciences, and Frontiers in Aging Neuroscience. Research fields span neural regeneration, traumatic brain injury, neuroinflammation and demyelination, depression, Rett syndrome, neuroprotection against oxidative stress, vascular cognitive impairment, Parkinson's disease, spinal cord injury, cardiac remodeling, and Alzheimer's disease.
| Species | Application | Title |
|---|---|---|
Bioact Mater Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury | ||
Nat Chem Biol SARM1 activation promotes axonal degeneration via a two-step phase transition | ||
Theranostics Implantation of regenerative complexes in traumatic brain injury canine models enhances the reconstruction of neural networks and motor function recovery. | ||
J Neuroinflammation Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelination | ||
J Ethnopharmacol Kai-Xin-San ameliorates fluoxetine-resistant depressive-like behaviors by modulating tryptophan-kynurenine metabolic homeostasis in a rodent model | ||
Biomater Sci 3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine. |
