NEK9 Antibody
Rabbit Polyclonal
Catalog number: 11192-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, human placenta tissue |
| Positive IHC detected in | human lymphoma |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 11192-1-AP(NEK9 Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human lymphoma using 11192-1-AP(NEK9 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NEK9 antibody 11192-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | NEK9 fusion protein ag1678 | Full name | NIMA (never in mitosis gene a)- related kinase 9 |
|
Calculated molecular weight |
107kd |
Observed molecular
weight |
120kd |
|
GenBank accession number |
BC009336 | Gene ID (NCBI) | 91754 |
| Gene symbol | NEK9 | Synonyms | DKFZp434D0935; MGC138306; MGC16714; NERCC; NERCC1; Nek8 |
Background
NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as aβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecularmass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704). This antibody is specific to NEK9. |
