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NFE2L3 Polyclonal antibody

NFE2L3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17745-1-AP

Synonyms

NF E2 related factor 3, NFE2 related factor 3, NFE2L3, NRF3

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using NFE2L3 Polyclonal antibody (17745-1-AP)

    WB analysis of human placenta using 17745-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17745-1-AP (NFE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFE2L3 Polyclonal antibody (17745-1-AP)

    IF Staining of HepG2 using 17745-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17745-1-AP (NFE2L3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17745-1-AP targets NFE2L3 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFE2L3 fusion protein Ag12005
Full Name nuclear factor (erythroid-derived 2)-like 3
Calculated Molecular Weight 694 aa, 76 kDa
Observed Molecular Weight 76 kDa
GenBank Accession NumberBC068455
Gene Symbol NFE2L3
Gene ID (NCBI) 9603
RRIDAB_2153211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NFE2L3 antibody 17745-1-APDownload protocol
IF protocol for NFE2L3 antibody 17745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Comput Methods Programs Biomed

Transcription factor NFE2L3 promotes the proliferation of esophageal squamous cell carcinoma cells and causes radiotherapy resistance by regulating IL-6

Authors - Tingting Chen
  • KD Validated
View Article
humanWB

J Oncol

miR-23b-3p Inhibits the Oncogenicity of Colon Adenocarcinoma by Directly Targeting NFE2L3.

Authors - Guohong Huang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tao (Verified Customer) (02-21-2019)

This antibody could detect a band at molecular weight of 130 kD.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Beas2B
NFE2L3 Antibody Western Blot, validation (1:1000 dilution) in Beas2B (Cat no:17745-1-AP)