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  • KD/KO Validated

NUDT16 Polyclonal antibody

NUDT16 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12889-1-AP

Synonyms

Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12889-1-AP targets NUDT16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT16 fusion protein Ag3925
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 16
Calculated Molecular Weight 195 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC031215
Gene Symbol NUDT16
Gene ID (NCBI) 131870
RRIDAB_10640536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUDT16 antibody 12889-1-APDownload protocol
IHC protocol for NUDT16 antibody 12889-1-APDownload protocol
IF protocol for NUDT16 antibody 12889-1-APDownload protocol
IP protocol for NUDT16 antibody 12889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.

Authors - Siddharth Shukla
  • KD Validated
WB

Mol Biol Cell

L-bodies are RNA-protein condensates driving RNA localization in Xenopus oocytes.

Authors - Christopher R Neil
humanIHC

Front Immunol

m7G regulator-mediated methylation modification patterns define immune cell infiltration and patient survival

Authors - Lu Wang