PALLD,palladin AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IP|
|Cited applications||IF, IHC, IP, WB|
|Species specificity||human; other species not tested.|
|Cited species||human, mouse, rat|
|Positive WB detected in||HeLa cells, HepG2 cells|
|Positive IHC detected in||human ovary tumor|
|Recommended dilution||WB: 1:1000-1:10000|
IP = Immunoprecipitation
- WB result of 10853-1-AP (PALLD antibody) on 293 cell.
- IP result of anti-PALLD(10853-1-AP for IP and Detection).
- HeLa cells were subjected to SDS PAGE followed by western blot with 10853-1-AP(PALLD,palladin Antibody) at dilution of 1:2000
- Immunohistochemistry of paraffin-embedded human ovary tumor using 10853-1-AP(PALLD,palladin Antibody) at Dilution 1:100 (under 10x lens)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||PALLD,palladin fusion protein ag1288||Full name||palladin, cytoskeletal associated protein|
|95 kDa, 140kDa|
|BC013867||Gene ID (NCBI)||23022|
|Gene symbol||PALLD||Synonyms||CGI-151; FLJ22190; FLJ38193; FLJ39139; KIAA0992; PNCA1; SIH002|
Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.
|16179-1-AP||PALLD,palladin-Specific antibody Rabbit Polyclonal|
|60167-1-Ig||PALLD,palladin-Specific antibody Mouse Monoclonal|