Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of L02 using 16217-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16217-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 16217-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 16217-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse liver using 16217-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse liver using 16217-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of L02 using 16217-1-AP
Immunofluorescent analysis of L02 cells using 16217-1-AP (PDGFRL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 16217-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDGFRL Polyclonal antibody (16217-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

PDGFRL Polyclonal antibody

Name: PDGFRL Polyclonal antibody
Brand: Proteintech
SKU: 16217-1-AP

PDGFRL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 16217-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PDGF receptor beta-like tumor suppressor, PDGFR like protein, PDGFR-like protein, PDGRL, Platelet-derived growth factor receptor-like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	L02 cells, mouse liver tissue
Positive IHC detected in	human liver cancer tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below

Product Information

16217-1-AP targets PDGFRL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9218 Product name: Recombinant human PDGFRL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-375 aa of BC010927 Sequence: MKVWLLLGLLLVHEALEDVTGQHLPKNKRPKEPGENRIKPTNKKVKPKIPKMKDRDSANSAPKTQSIMMQVLDKGRFQKPAATLSLLAGQTVELRCKGSRIGWSYPAYLDTFKDSRLSVKQNERYGQLTLVNSTSADTGEFSCWVQLCSGYICRKDEAKTGSTYIFFTEKGELFVPSPSYFDVVYLNPDRQAVVPCRVTVLSAKVTLHREFPAKEIPANGTDIVYDMKRGFVYLQPHSEHQGVVYCRAEAGGRSQISVKYQLLYVAVPSGPPSTTILASSNKVKSGDDISVLCTVLGEPDVEVEFTWIFPGQKDERPVTIQDTWRLIHRGLGHTTRISQSVITVEDFETIDAGYYICTAQNLQGQTTVATTVEFS Predict reactive species
Full Name	platelet-derived growth factor receptor-like
Calculated Molecular Weight	375 aa, 42 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC010927
Gene Symbol	PDGFRL
Gene ID (NCBI)	5157
RRID	AB_2878230
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15198
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for PDGFRL antibody 16217-1-AP	Download protocol
IF protocol for PDGFRL antibody 16217-1-AP	Download protocol
IHC protocol for PDGFRL antibody 16217-1-AP	Download protocol
WB protocol for PDGFRL antibody 16217-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution Authors - Michael T H Ng View Article
human	WB	Aging (Albany NY) Identification of a unique stress response state of T cells-related gene signature in patients with gastric cancer Authors - Qin Yang KD Validated View Article
human	IHC	Mol Carcinog The cumulative antitumor effects of regorafenib and radiotherapy in hepatocellular carcinoma Authors - Shuwen Kuang View Article