PDIA1 Antibody
Rabbit Polyclonal
Catalog number: 11245-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, human thyroid gland tissue |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11245-1-AP(PDIA1 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human prostate cancer using 11245-1-AP(PDIA1 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using P4HB antibody 11245-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PDIA1 fusion protein ag1747 | Full name | prolyl 4-hydroxylase, beta polypeptide |
|
Calculated molecular weight |
57kd |
Observed molecular
weight |
57kd |
|
GenBank accession number |
BC014504 | Gene ID (NCBI) | 5034 |
| Gene symbol | P4HB | Synonyms | DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB |
Background
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).This antibody is specific to PDIA1. |
