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  • KD/KO Validated

PHLDA2 Polyclonal antibody

PHLDA2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14661-1-AP

Synonyms

BRW1C, BWR1C, HLDA2, IPL, p17 BWR1C, PHLDA2, TSSC3



Tested Applications

Positive WB detected inL02 cells, human placenta tissue, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14661-1-AP targets PHLDA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, Goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLDA2 fusion protein Ag6331
Full Name pleckstrin homology-like domain, family A, member 2
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC005034
Gene Symbol PHLDA2
Gene ID (NCBI) 7262
RRIDAB_2163294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PHLDA2 antibody 14661-1-APDownload protocol
IHC protocol for PHLDA2 antibody 14661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.

Authors - Yuji Kubota
humanWB

Aging (Albany NY)

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.

Authors - Zhan Ma
  • KD Validated
humanWB

Pathol Res Pract

FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells

Authors - Guangyi Liu
  • KD Validated
GoatWB

Gene

Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta.

Authors - Ruihong Wang
humanIHC,WB

Sci Rep

Characteristics of lactate metabolism phenotype in hepatocellular carcinoma

Authors - Jiacheng Zhang