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PIM2 Polyclonal antibody

PIM2 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25865-1-AP

Synonyms

pim 2 oncogene, Pim 2h, PIM2

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of Raji cells using PIM2 Polyclonal antibody (25865-1-AP)

    IP experiment of Raji using 25865-1-AP

    IP result of anti-PIM2 (IP:25865-1-AP, 4ug; Detection:25865-1-AP 1:300) with Raji cells lysate 1320ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PIM2 Polyclonal antibody (25865-1-AP)

    IHC staining of mouse liver using 25865-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25865-1-AP (PIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IP detected inRaji cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25865-1-AP targets PIM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIM2 fusion protein Ag23130
Full Name pim-2 oncogene
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC018111
Gene Symbol PIM2
Gene ID (NCBI) 11040
RRIDAB_2880275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIM2 was first identified in T and B cell lymphomas in mice and is a member of a serine/threonine kinase family of proto-oncogenes, which also includes PIM1 and PIM3 (PMID:24505470). They share high sequence homology at the amino acid level; PIM1 and PIM2 are 61% identical and PIM1 and PIM3 are 71% identical (PMID:34503111). The PIM2 protein plays an important role in promoting cell survival and preventing apoptosis. PIM2 is mainly expressed in lymphoid and brain tissues. In human cells, the PIM2 kinase has only two isoforms (34 and 41 kDa), but in mice there are there (34, 37, and 40 kDa), and the 34 kDa isoform has been the main focus of many studies because it plays a crucial role in tumor progression (PMID:33854618).

Protocols

Product Specific Protocols
IHC protocol for PIM2 antibody 25865-1-APDownload protocol
IP protocol for PIM2 antibody 25865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Phytomedicine

Abrine promotes cell proliferation and inhibits apoptosis of interleukin-1β-stimulated chondrocytes via PIM2/VEGF signalling in osteoarthritis.

Authors - Yong Meng
View Article