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  • Featured Product
  • KD/KO Validated

PISD Polyclonal antibody

PISD Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16401-1-AP

Synonyms

DJ858B16, PISD, PSDC, PSSC

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of K-562 using 16401-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of NIH/3T3 using 16401-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of L02 using 16401-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of L02 using 16401-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of L02 using 16401-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of human spleen using 16401-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of human kidney using 16401-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of COLO 320 using 16401-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of HeLa using 16401-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PISD Polyclonal antibody (16401-1-AP)

    WB analysis of HepG2 using 16401-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using PISD Polyclonal antibody (16401-1-AP)

    IP experiment of L02 using 16401-1-AP

    IP result of anti-PISD (IP:16401-1-AP, 3ug; Detection:16401-1-AP 1:500) with L02 cells lysate 1400ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PISD Polyclonal antibody (16401-1-AP)

    IHC staining of human kidney using 16401-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PISD Polyclonal antibody (16401-1-AP)

    IHC staining of human kidney using 16401-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inK-562 cells, COLO 320 cells, HeLa cells, HepG2 cells, human kidney tissue, human spleen tissue, L02 cells, NIH/3T3 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16401-1-AP targets PISD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PISD fusion protein Ag9499
Full Name phosphatidylserine decarboxylase
Calculated Molecular Weight375aa,43 kDa; 408aa,46 kDa
Observed Molecular Weight 50~55 kDa
GenBank Accession NumberBC001482
Gene Symbol PISD
Gene ID (NCBI) 23761
RRIDAB_2186673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

Protocols

Product Specific Protocols
WB protocol for PISD antibody 16401-1-APDownload protocol
IHC protocol for PISD antibody 16401-1-APDownload protocol
IP protocol for PISD antibody 16401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.

Authors - Yawen Ma
View Article
human

Front Cell Dev Biol

Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning

Authors - Yaoyao Song
View Article
humanWB

J Clin Biochem Nutr

Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition

Authors - Nobuhiko Uchida
View Article
humanWB

Cell Stem Cell

The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.

Authors - Ayesh K Seneviratne
  • KO Validated
View Article