PKM Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF7 cells, mouse brain tissue, mouse liver tissue, mouse skeletal muscle tissue, NIH/3T3 cells, Raji cells, SH-SY5Y cells, SKOV-3 cells |
| Recommended dilution | WB: 1:500-1:5000 |
IP = Immunoprecipitation
WB result of 10078-2-AP (PKM antibody) with various lysates.
HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP(PKM Antibody) at dilution of 1:1000
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | PKM fusion protein ag0110 | Full name | PKM2 pyruvate kinase, muscle |
|
Calculated molecular weight |
58kd |
Observed molecular
weight |
58 kDa |
|
GenBank accession number |
BC000481 | Gene ID (NCBI) | 5315 |
| Gene symbol | PKM2 | Synonyms | PK3; PKM; TCB; OIP3; CTHBP; THBP1 |
PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms . This antibody can recognize both PK-M1 and PK-M2. |
