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Prokineticin 1 Polyclonal antibody

Prokineticin 1 Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15152-1-AP

Synonyms

EG VEGF, EGVEGF, Mambakine, PK1, PRK1, PROK1, prokineticin 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of mouse kidney using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of mouse kidney using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of mouse liver using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of mouse liver using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IHC staining of mouse kidney using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    IF Staining of NIH/3T3 using 15152-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Prokineticin 1 antibody (15152-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Prokineticin 1 Polyclonal antibody (15152-1-AP)

    FC experiment of NIH/3T3 using 15152-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Prokineticin 1 (15152-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15152-1-AP targets Prokineticin 1 in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Prokineticin 1 fusion protein Ag5098
Full Name prokineticin 1
Calculated Molecular Weight 12 kDa
GenBank Accession NumberBC025399
Gene Symbol PROK1
Gene ID (NCBI) 84432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prokineticin 1 (PROK1) is also named as EG-VEGF and Mambakine, belongs to the to the AVIT (prokineticin) family. Prokineticin signaling comprises two secreted proteins (Prok-1 and Prok-2) and two cognate G-protein coupled receptors (PK-R1 and PK-R2) that are widely expressed in different tissues and of great versatility. Prokineticins were shown to promote angiogenesis in steroidgenic glands, heart and reproductive organs (PMID:18440852). PROK1 has been described as a secretory protein with pleiotropic functions and as a novel tissue-specific angiogenic factor (PMID:32355954). EG-VEGF/PK-1, described as selective angiogenic mitogen, is widely expressed in different tissues including steroidogenic endocrine glands (PMID:16320832). A lot of data suggests EG-VEGF to be restricted to endocrine glands and to some endocrine-dependent organs (PMID:28386275).

Protocols

Product Specific Protocols
IHC protocol for Prokineticin 1 antibody 15152-1-APDownload protocol
IF protocol for Prokineticin 1 antibody 15152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols