Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 14022-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14022-1-AP
WB result of PSMA8 antibody (14022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.
WB analysis of K-562 using 14022-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14022-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14022-1-AP
Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody 14022-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HepG2 using 14022-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA8 Polyclonal antibody (14022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

PSMA8 Polyclonal antibody

Name: PSMA8 Polyclonal antibody
Brand: Proteintech
SKU: 14022-1-AP

PSMA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14022-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha4s, Proteasome alpha 4 subunit, Proteasome subunit alpha-type 7-like, Proteasome subunit alpha-type 8, PSMA7L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells, mouse thymus tissue, HeLa cells, rat thymus tissue
Positive IHC detected in	human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

14022-1-AP targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5138 Product name: Recombinant human PSMA8 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-250 aa of BC042820 Sequence: MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVVINRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTEDAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEKEEAEKKKSKKSV Predict reactive species
Full Name	proteasome (prosome, macropain) subunit, alpha type, 8
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC042820
Gene Symbol	PSMA8
Gene ID (NCBI)	143471
RRID	AB_10896314
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TAA3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for PSMA8 antibody 14022-1-AP	Download protocol
IF protocol for PSMA8 antibody 14022-1-AP	Download protocol
IHC protocol for PSMA8 antibody 14022-1-AP	Download protocol
WB protocol for PSMA8 antibody 14022-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis. Authors - Zhiming Li View Article
mouse	IF	PLoS Genet The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. Authors - Laura Gómez-H View Article
		Zool Res Both 20S and 19S proteasome components are essential for meiosis in male mice Authors - Ting-Ting Han View Article
mouse	WB	Sci China Life Sci Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice Authors - Yiling Zhou View Article