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PYGL Polyclonal antibody

PYGL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15851-1-AP

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, mouse heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15851-1-AP targets PYGL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYGL fusion protein Ag8664
Full Name phosphorylase, glycogen, liver
Calculated Molecular Weight 846 aa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC009895
Gene Symbol PYGL
Gene ID (NCBI) 5836
RRIDAB_2175014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PYGL antibody 15851-1-APDownload protocol
IHC protocol for PYGL antibody 15851-1-APDownload protocol
IF protocol for PYGL antibody 15851-1-APDownload protocol
IP protocol for PYGL antibody 15851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes.

Authors - Sophia Maschalidi

Nat Med

PMID: 30150719

Authors - Roger W Hunter
humanWB

Cell Metab

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Authors - Marion Curtis
human,mouseWB

Cell Metab

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Authors - Phyu M Thwe
mouseWB

J Clin Invest

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

Authors - Miguel A Lanaspa
humanIHC,IF,WB

Int J Biol Sci

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer

Authors - Qian Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer