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pregnancy zone protein Polyclonal antibody

pregnancy zone protein Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21742-1-AP

Synonyms

CPAMD6, pregnancy zone protein, PZP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    WB analysis using 21742-1-AP

    Human plasma were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    WB analysis of human placenta using 21742-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    WB analysis of human brain using 21742-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    IHC staining of human placenta using 21742-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    IHC staining of human placenta using 21742-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    IHC staining of human brain using 21742-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using pregnancy zone protein Polyclonal antibody (21742-1-AP)

    IHC staining of human brain using 21742-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman plasma, human placenta tissue, human brain tissue
Positive IHC detected inhuman placenta tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21742-1-AP is pregnancy zone protein Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen pregnancy zone protein fusion protein Ag16471
Full Name pregnancy-zone protein
Calculated Molecular Weight 1482 aa, 164 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberBC111756
Gene Symbol PZP
Gene ID (NCBI) 5858
RRIDAB_10732823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pregnancy Zone Protein (PZP) is an immunosuppressive protein that is expressed by the placenta. The PZP is a thiol ester-containing alpha macroglobulin protein that controls the proteolytic pathways during pregnancy and contributes to maintaining the immunologically privileged status of the fetus. Recently PZP over-expression has been described as a novel biomarker for predicting the prognosis of hepatocellular carcinoma and its high expression has been reported in solid tumors.

Protocols

Product Specific Protocols
WB protocol for pregnancy zone protein antibody 21742-1-APDownload protocol
IHC protocol for pregnancy zone protein antibody 21742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Proteomics

iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.

Authors - Hongmei Lu
View Article