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  • KD/KO Validated

RBM3 Polyclonal antibody

RBM3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14363-1-AP

Synonyms

IS1 RNPL, Putative RNA binding protein 3, RBM3, RNA binding motif protein 3, RNPL



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, A375 cells, Raji cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue, human lung tissue, human brain tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14363-1-AP targets RBM3 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM3 fusion protein Ag5934
Full Name RNA binding motif (RNP1, RRM) protein 3
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC006825
Gene Symbol RBM3
Gene ID (NCBI) 5935
RRIDAB_2269266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).

Protocols

Product Specific Protocols
WB protocol for RBM3 antibody 14363-1-APDownload protocol
IHC protocol for RBM3 antibody 14363-1-APDownload protocol
IF protocol for RBM3 antibody 14363-1-APDownload protocol
IP protocol for RBM3 antibody 14363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.

Authors - Diego Peretti
  • KD Validated
human,squirrelWB

Cell

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.

Authors - Jingxing Ou

EBioMedicine

The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.

Authors - Wei Dong
humanWB

Clin Transl Med

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Authors - Qi Wen Chen
mouseWB

J Cell Biol

Cold-induced protein RBM3 orchestrates neurogenesis via modulating Yap mRNA stability in cold stress.

Authors - Wenlong Xia
  • KD Validated
mouseWB

Front Cell Dev Biol

The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.

Authors - Jingyi Yan