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  • Featured Product
  • KD/KO Validated

RHCG Polyclonal antibody

RHCG Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 11949-1-AP

Synonyms

Ammonium transporter Rh type C, C15orf6, CDRC2, PDRC2, Rh family type C glycoprotein, Rh family, C glycoprotein, Rh glycoprotein kidney, Rh type C glycoprotein, RHCG, RHGK, SLC42A3, Tumor related protein DRC2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RHCG Polyclonal antibody (11949-1-AP)

    WB analysis of HEK-293 using 11949-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    WB analysis of human kidney using 11949-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human pancreas cancer using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RHCG Polyclonal antibody (11949-1-AP)

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11949-1-AP targets RHCG in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHCG fusion protein Ag2493
Full Name Rh family, C glycoprotein
Calculated Molecular Weight 479 aa, 53 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC030965
Gene Symbol RHCG
Gene ID (NCBI) 51458
RRIDAB_2269482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RHCG antibody 11949-1-APDownload protocol
IHC protocol for RHCG antibody 11949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,CoIP

Kidney Int

The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.

Authors - Soline Bourgeois
  • KO Validated
View Article
humanWB,IHC

Aging (Albany NY)

Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.

Authors - Wenguang Xu
View Article