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  • KD/KO Validated

ROMO1 Polyclonal antibody

ROMO1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24200-1-AP

Synonyms

C20orf52, MTGMP, Protein MGR2 homolog, ROMO1, ROS modulator 1



Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24200-1-AP is ROMO1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROMO1 fusion protein Ag20368
Full Name reactive oxygen species modulator 1
Calculated Molecular Weight 79 aa, 8 kDa
GenBank Accession NumberBC008488
Gene Symbol ROMO1
Gene ID (NCBI) 140823
RRIDAB_2879453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ROMO1 antibody 24200-1-APDownload protocol
IHC protocol for ROMO1 antibody 24200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis

Authors - Jiping Yao
  • KD Validated
ratWB

Neurochem Res

NL-1 Promotes PINK1-Parkin-Mediated Mitophagy Through MitoNEET Inhibition in Subarachnoid Hemorrhage

Authors - Tongyu Zhang
pigWB,IF

Cell Div

ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs.

Authors - Dongjie Zhou
  • KD Validated
humanWB

J Nanobiotechnology

Metal-organic framework-encapsulated dihydroartemisinin nanoparticles induces apoptotic cell death in ovarian cancer by blocking ROMO1-mediated ROS production

Authors - Yuanliang Yan
humanWB

J Cell Biol

ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.

Authors - Frank Richter
  • KD Validated