Tested Applications
| Positive IHC detected in | mouse lung tissue, human lung cancer tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10490-1-AP targets Uteroglobin/CC10 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0758 Product name: Recombinant human Uteroglobin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-91 aa of BC004481 Sequence: MKLAVTLTLVTLALCCSSASAEICPSFQRVIETLLMDTPSSYEAAMELFSPDQDMREAGAQLKKLVDTLPQKPRESIIKLMEKIAQSSLCN Predict reactive species |
| Full Name | secretoglobin, family 1A, member 1 (uteroglobin) |
| Calculated Molecular Weight | 10 kDa |
| GenBank Accession Number | BC004481 |
| Gene Symbol | Uteroglobin |
| Gene ID (NCBI) | 7356 |
| RRID | AB_2183285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Uteroglobin/CC10 antibody 10490-1-AP | Download protocol |
| IF protocol for Uteroglobin/CC10 antibody 10490-1-AP | Download protocol |
| IHC protocol for Uteroglobin/CC10 antibody 10490-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Uteroglobin/CC10 polyclonal antibody (Cat# 10490-1-AP) has 41 citations published in journals including Nature, Cell, Lancet Respiratory Medicine, Nature Genetics, European Respiratory Journal, Cell Stem Cell, Nature Communications, Theranostics, Molecular Therapy, Developmental Cell, JCI Insight, Scientific Reports, and others. Associated research fields encompass pulmonary biology, SARS-CoV-2 and viral infections, lung fibrosis, COPD, asthma, lung carcinogenesis, acute lung injury, pulmonary hypertension, immune responses, developmental biology, and toxicology.
| Species | Application | Title |
|---|---|---|
Cell A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates | ||
Lancet Respir Med Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures. | ||
Eur Respir J Eosinophils protect against pulmonary hypertension through 14-HDHA and 17-HDHA | ||
Eur Respir J Tropism of influenza B viruses in human respiratory tract explants and airway organoids. |
Reviews
What customers say
Both reviewers report strong performance. One user (5/5) found the antibody worked very well for both IFA and IHC on paraffin-embedded mouse lung sections at 1:200 dilution. The other (4/5) used it at 1:500 on human fetal lung tissue, noting specific staining with low background, and observed that it performed better in cryosections without antigen retrieval. Overall, users highlight reliable specificity, low background, and flexibility across tissue types and applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rashmi (Verified Customer) (09-18-2025) | The antibody worked very well for both IFA and IHC staining performed on paraffin-embedded mouse lung sections.
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Tessa (Verified Customer) (02-03-2019) | Used in 20pcw human fetal lung and got nice staining. Looks very specific with low background. Found that it did not require antigen retrieval in cryosections. In fact it turned out better in sections where 10mM Na Citrate buffer at pH6 was not used.
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